Abstract
Three species of botryosphaeriaceous fungi,Botryosphaeria sp. isolate MAMB-5,Botryosphaeria ribis andLasiodiplodia theobromae, were compared for the production of pycnidia and laccases. Laccases were produced both intra- and extra-cellularly when the fungi were cultivated on basal medium in the presence and absence of veratryl alcohol, withBotryosphaeria sp. MAMB-5 showing the highest enzyme titres. Electrophoretic examination of intracellular marker proteins (esterases and phosphatases) and laccases indicated that the three species were genetically distinctly different, although the laccase zymograms for the three fungi showed similarity. The production of pycnidia occurred under continuous lighting at 28°C, but conditions differed among the three fungal species. Production could be induced on artificial media (potato-dextrose and oat agar) under stress-induced conditions where the mycelium was stimulated by physical abrasion, and in the case ofBotryosphaeria sp. isolate MAMB-5 on eucalypt woodchips. Evidence is presented that veratryl alcohol facillitated the secretion of intracellular-localised laccases into the extracellular medium.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
