Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy

Abstract

Red blood cells of vertebrates have undergone evolutionary changes over time, leading to the diversification of morphological and mechanical properties of red blood cells (RBCs). Among the vertebrates, camelids have the most different RBC characteristics. As a result of adaptation to the desert environment, camelid RBCs can expand twice as much of their total volume in the case of rapid hydration yet are almost undeformable under mechanical stress. In this work, the mechanical and chemical differences in the RBC properties of the human and camelid species were examined using optical tweezers and Raman spectroscopy. We measured the deformability of camel and human RBCs at the single-cell level using optical tweezers. We found that the deformability index (DI) of the camel and the human RBCs were 0.024 ± 0.019 and 0.215 ± 0.061, respectively. To investigate the chemical properties of these cells, we measured the Raman spectra of the whole blood samples. The result of our study indicated that some of the Raman peaks observed on the camel’s blood spectrum were absent in the human blood’s spectrum, which further points to the difference in chemical contents of these two species’ RBCs.