Comparison of the growth characteristics of influenza subtype H10 reassortant viruses in different cells

Abstract

Objective To evaluate the cells for producing vaccine of avian influenza H10 subtype, the growth characteristics of influenza H10 subtype reassortant viruses in different cells (MDCK and Vero) were investigated. Methods Reassortant viruses, RG-H10N1(7+ 1) and RG-H10N8(6+ 2), between wild-type virus A/Jiangxi-donghu/346/2013(JXH10N8) and high-yielding virus A/ Puerto Rico/ 8/ 34(PR8) were constructed with reverse genetic system. Viruses were propagated in SPF embryonated chicken eggs. The growth characteristics of the two reassortant viruses were compared after inoculating MDCK and Vero cells respectively, with RG-PR8 as the control. Viral growth characteristics were studied at different MOI in MDCK cells. Results All viruses could be detected TCID50 in MDCK cells. RG-PR8 and RG-H10N1could be detected TCID50 in Vero cells but not RG-H10N8. There were no significant difference in HA titer between the two reassortant viruses at the same MOI, but both reassortant viruses exhibited lower HA titers than that of PR8 in MDCK cells. Conclusion Both influenza H10 subtype reassortant viruses grew better in MDCK cells. MDCK cells are more sensitive to the influenza H10 reassortant viruses, and NA from different subtypes could affect the grow capability of reassortant viruses in Vero cells. Key words: Influenza virus; H10 subtype; Reassortant virus; Growth characteristics