Abstract
The authors evaluated four disinfectant pre-impregnated wipes (DPW) for efficacy against Ebola virus Makona variant (EBOV) and vesicular stomatitis virus (VSV), Indiana serotype. Steel carriers were inoculated with the infectious virus and then were wiped with DPW in the Wiperator instrument per ASTM E2967-15. Following the use of J-Cloth impregnated with medium (negative control wipes) or the use of activated hydrogen peroxide (AHP)-, ethanol-, sodium hypochlorite (NaOCl)-, or single or dual quaternary ammonium compound (QAC)-based DPW, virus recovery from the carriers was assayed by titration assay and by two passages on Vero E6 cells in 6-well plates. The Wiperator also enabled the measurement of potential transfer of the virus from the inoculated carrier to a secondary carrier by the DPW or control wipes. The J-Cloth wipes wetted with medium alone (no microbicidal active) removed 1.9–3.5 log10 of virus from inoculated carriers but transferred ~4 log10 of the wiped virus to secondary carriers. DPW containing AHP, ethanol, NaOCl, or single or dual QAC as active microbicidal ingredients removed/inactivated ~6 log10 of the virus, with minimal EBOV or no VSV virus transfer to a secondary surface observed. In Ebola virus outbreaks, a DPW with demonstrated virucidal efficacy, used as directed, may help to mitigate the unintended spread of the infectious virus while performing surface cleaning.
Highlights
Disinfectant pre-impregnated wipes (DPW) are an optional intervention for disrupting the cycle of infection transmission since these wipes may remove and inactivate pathogens from contaminated high-touch environmental surfaces (HITES) during use [1]
The results obtained during carrier testing of the control wipes and five different disinfectant pre-impregnated wipes (DPW) are displayed in Tables 1, 2 for Ebola virus Makona variant (EBOV) and Tables 3, 4 for vesicular stomatitis virus (VSV), respectively
The results of the Wiperator study performed to evaluate the efficacy of DPW for decontaminating EBOV-inoculated carriers and VSVinoculated carriers are displayed in Tables 1, 3, respectively
Summary
Disinfectant pre-impregnated wipes (DPW) are an optional intervention for disrupting the cycle of infection transmission since these wipes may remove and inactivate pathogens from contaminated high-touch environmental surfaces (HITES) during use [1]. As demonstrated previously [2,3,4,5,6,7], a wipe that is not impregnated with an effective disinfectant may provide physical removal only Such a wipe, once used, may spread pathogens from a wiped contaminated surface to a secondary non-contaminated surface. Once used, may spread pathogens from a wiped contaminated surface to a secondary non-contaminated surface For this reason, wipes that do not contain a microbicidal activity with sufficient efficacy against the target pathogen should not represent an effective intervention for limiting the spread of an infectious agent caused by indirect transmission from contaminated HITES
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