Comparison of the effects of triamcinolone acetonide or platelet-rich plasma on expression of extracellular matrix-related genes in equine healthy chondrocytes in vitro

Heloisa Einloft Palma,Karin Erica Brass,Alfredo Quites Antoniazzi,Camila Cantarelli,Alexandre Krause,Luciana Maria Fontanari Krause,Patrícia Wolkmer,Julien Wergutz,Gabriele Biavaschi Da Silva,Miguel Gallio,Flavio Desessards De La Corte,Kalyne Bertolin

doi:10.1590/0103-8478cr20180262

Abstract

ABSTRACT: In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group when the expressions of MMP9, MMP13, IL-6 and ACAN genes were evaluated (P<0.05). With these results, we can suggest that the treatments were not deleterious to equine cultured chondrocyte, once they did not stimulate MMPs and IL-6 synthesis or caused changes in ACAN.

Highlights

Articular cartilage is composed of chondrocytes embedded in an extracellular matrix (ECM) formed mainly by type II collagen and proteoglycan aggrecan molecules, which they synthesize and degrade
Because chondrocytes are responsible for maintaining cartilage homeostasis, the use of substances that could damage the chondrocytes should be avoided
No differences (P

Summary

INTRODUCTION

Articular cartilage is composed of chondrocytes embedded in an extracellular matrix (ECM) formed mainly by type II collagen and proteoglycan aggrecan molecules, which they synthesize and degrade. V.49, n.7, Necrosis Factor alpha (TNF-α) and Interleukin-6 (IL 6) are pro-inflammatory cytokines involved in the pathophysiology of OA, inducing overexpression of metalloproteinases (MMPs) and decrease synthesis of macromolecules, including aggrecan (PORÉE et al, 2008; KAPOOR et al, 2011). Developing strategies for intra-articular therapies in horses that promote tissue regeneration is very important, and, for this, platelet-rich plasma (PRP) has been a potentially regenerative therapy (TEXTOR & TABLIN, 2013) It is considered a good therapeutic alternative because it is a cheap technique and with a low possibility of reactions by the recipient, since its own biological material is used (HUR et al, 2014). Our study aimed to analyze the potential interference of administration of TA and PRP in culture of healthy chondrocytes, extracted from healthy metacarpophalangeal joints of horses, in relation to the gene expression of MMP13 and MMP9, IL-6 and ACAN

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION