Abstract
Phosphofructokinase activity in prepubertal rat ovaries is elevated by in vitro treatment with human chorionic gonadotropin or luteinizing hormone. The stimulatory effects of the two gonadotropins are additive. Puromycin and actinomycin D do not affect the enzyme increase induced by human chorionic gonadotropin, but the stimulatory action of luteinizing hormone is completely abolished by these antibiotics. These data suggest that the two hormones have different mechanisms of action and probably occupy different receptor sites on the ovarian cells.
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