Abstract
The aim of this study was to compare the effect of pharmacologic inhibition of 3 different catalytic domains of fatty acid synthase (FASN) on the cell cycle and apoptosis and associate with the morphologic alterations in squamous cell carcinoma 9 (SCC-9) cells. SCC-9 cells were treated with C75, triclosan (TCS), and orlistat (ORL) (inhibitors of KS, ER, and TE domains of FASN) for 24 to 48 hours and analyzed by flow cytometry and phase contrast microscopy. FASN inhibition with C75, ORL means Orlistat; TCS means Triclosan induced different effects on the morphology of SCC-9 cells. Cells treated with ORL showed a fusiform pattern. Instead, TCS induced the formation of cytoplasmic granules and C75 promoted a decrease of cell area. Also, the treatments reduced the number of cells in the S phase and increased the number of cells in the G0/G1 phase. Also, we observed an increase of apoptotic cells and a decrease of viable cells, which was observed in 3 different concentrations of all the drugs. However, TCS showed higher effectiveness in the apoptosis of SCC-9 cells. The morphologic changes of each inhibitor were linked not only to the specific inhibition of FASN activity but also could be due to adverse effects caused by the drugs. FAPESP.
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