Abstract
Colonization of <i>Azotobacter chroococcum</i> Mac 27 strain on wheat roots was monitored using double antibody sandwich enzyme linked immunosorbent assay (DAS ELISA) and immuno-fluorescence in terms of colonization sites, migration and survival of the bacteria. Furthermore, the effectivity of inoculation of <i>A. chroococcum</i> and <i>Pantoea agglomerans</i> D5/23 strain on wheat plant parameters under greenhouse conditions was investigated. Studies with DAS ELISA revealed that root tip had significant titre of inoculants as compared to the basal root parts. <i>A. chroococcum</i> colonized roots as well as soil and also migrated along with roots. An overall increase in plant growth was observed with inoculation of these bacteria. Both <i>A. chroococcum</i> and <i>P. agglomerans</i> were found to increase plant dry matter, nitrogen and phosphate uptake.
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