Abstract
Three Solanaceae hosts (TSHs), S. tuberosum, N. benthamiana and S. lycopersicum, represent the three major phylogenetic clades of Solanaceae plants infected by Phytophthora infestans, which causes late blight, one of the most devastating diseases seriously affecting crop production. However, details regarding how different Solanaceae hosts respond to P. infestans are lacking. Here, we conducted RNA-seq to analyze the transcriptomic data from the TSHs at 12 and 24 h post P. infestans inoculation to capture early expression effects. Macroscopic and microscopic observations showed faster infection processes in S. tuberosum than in N. benthamiana and S. lycopersicum under the same conditions. Analysis of the number of genes and their level of expression indicated that distinct response models were adopted by the TSHs in response to P. infestans. The host-specific infection process led to overlapping but distinct in GO terms and KEGG pathways enriched for differentially expressed genes; many were tightly linked to the immune response in the TSHs. S. tuberosum showed the fastest response and strongest accumulation of reactive oxygen species compared with N. benthamiana and S. lycopersicum, which also had similarities and differences in hormone regulation. Collectively, our study provides an important reference for a better understanding of late blight response mechanisms of different Solanaceae host interactions.
Highlights
To further study the differences among the Three Solanaceae hosts (TSHs) in response to infection, leaves inoculated with zoospores of P. infestans at among the TSHs in response to infection, leaves inoculated with zoospores of P. infestans and24
The results showed that a higher number of differentially expressed genes (DEGs) were and S. lycopersicum, indicating that S. tuberosum had the fastest response rate during the expressed during the early stage in S. tuberosum at 12 hpi than that in N. benthamiana and early stage of pathogen infection
The results suggested that the infection process of P. infestans in Solanaceae hosts transcriptional changes at the early timepoints compared to N. benthamiana and S. lycoperpresented is somewhat host-specific
Summary
With approximately 1500 species, is the largest plant family in the Solanaceae and includes economically important species, for example, potato (Solanum tuberosum L.) and tomato (Solanum lycopersicum), as well as model tobacco plants Nicotiana benthamiana [1,2]. As the world’s third most important food crop, potato has high nutritional value and plays an important role in enhancing global food security [3]. Tomato is a vital horticultural and economic crop cultivated worldwide [4]. N. benthamiana, has been widely employed for studies of host-pathogen interaction due to its susceptibility to many relevant pathogens [5,6]. Despite being relatively closely related species, the genomes of the three
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