Abstract
Determination of renin plasma levels is useful in the diagnosis of hypertension and in the therapeutic follow-up of hypertensive patients. Plasmatic concentration of renin decreases in patients with hypertension due to a primary hyperaldosteronism, contrary to renovascular hypertension where concentrations of renin and aldosterone are both elevated. Blood samples (serum, EDTA plasma) were analysed using two different chemiluminiscent methods CLIA LIAISON® and radioimmunoassay for aldosterone (IMMUNOTECH Beckman Coulter) and renin (Cisbio Bioassay) measurements were compared. We used both methods to ascertain the correlation between serum vs. EDTA plasma levels of aldosterone (RIA, CLIA) and renin (IRMA, CLIA) and to compare aldosterone to renin ratios for CLIA and for radioimmunoassay: serum aldosterone to plasma renin and plasma aldosterone to plasma renin. We compared serum aldosterone CLIA vs. RIA (rP=0.933, P<0.001) and plasma renin determined using CLIA vs. IRMA (rP=0.965, P=0.062). Furthermore, we used both methods to establish the correlation between the serum vs. plasma levels of aldosterone: RIA (rP=0.980, P<0.001); CLIA (rP=0.994, P=0.353) and serum vs. plasma levels of renin: IRMA (rP=0.948, P<0.001); CLIA (rP=0.921, P=0.011). Aldosterone (serum, plasma) to plasmatic renin ratios for CLIA (rP=0.999, P=0.286) and for radioimmunoassay (rP=0.992, P=0.025). Our data demonstrate that renin and aldosterone concentrations obtained using CLIA correlate with renin and aldosterone concentrations using radioimmunoassay methods. Correlation coefficients of pair results ranged from 0.921 to 0.994. Aldosterone (serum, EDTA plasma) to plasmatic renin ratios are comparable and any of them can be used with no significant differences found.
Highlights
Primary hyperaldosteronism (PH) is nowadays the most frequent form of secondary endocrine-mediated hypertension
Serum aldosterone levels using CLIA are lower in comparison with serum aldosterone levels using RIA
We found the significant effect of sample material, except of aldosterone determination using CLIA
Summary
Primary hyperaldosteronism (PH) is nowadays the most frequent form of secondary endocrine-mediated hypertension. Measurement of serum aldosterone in conjunction with plasma renin and their ratio are used clinically to differentiate between primary and secondary hyperaldosteronism (Trenkel et al, 2002; Reincke et al, 2003; Barigou et al, 2015). Our tests on two analytical systems were mainly focused on comparing the results of serum aldosterone (RIA vs CLIA) and plasma renin (IRMA vs CLIA). Parallel sample testing was conducted using both analytical methods, using the LIAISON® automated immunoanalyzer (CLIA) and the STRATEC SR 300 automated immunoanalyzer (RIA, IRMA). For both analytical systems (CLIA, RIA or IRMA) we compared the ratios: serum aldosterone to plasmatic renin and plasmatic aldosterone to plasmatic renin
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