Comparison of the Antioxidan Activity of the Ethanol Extract and Ethyl Acetate Fraction of Field Pavey (Nasturtium Montanum) Using the DPPH Method (1,1-diphenyl-2-2- picrylhydrazyl)

Abstract

Antioxidants are substances that inhibit reactions by free radicals. One example of natural antioxidants is field mustard leaves. The use of field mustard leaves (Nasturtium montanum) in the manufacture of ethyl acetate fraction and its antioxidant activity is not yet known with certainty. Therefore, it is necessary to conduct research on the antioxidant activity of the ethyl acetate fraction of mustard greens (Nasturtium montanum) leaves.This research aims to determine the antioxidant activity in the ethyl acetate fraction of field mustard greens (Nasturtium montanum) using the DPPH (1,1-diphenyl-2-2-picrylhydrazyl) method. Ethyl acetate fraction samples were prepared with 4 concentration series of 40,60,80,100 ppm. Pipette 2 mL of each concentration and add 2 mL of 50 ppm DPPH. Then incubate for 30 minutes. Absorbance was measured using a UV-Vis spectrophotometer at λ 517 nm and expressed as an IC50 (Inhibitory Concentration) value which shows the concentration of an antioxidant compound that results in the capture of 50% of DPPH radicals. The results of the antioxidant activity test of the ethyl acetate fraction of field mustard greens (Nasturtium montanum) showed that there was antioxidant activity with an IC50 value of 98.57 µg/mL, classified as strong, 50-100 µg/mL. Based on this study, field mustard leaves (Nasturtium montanum) have strong antioxidant activity.