Comparison of Syzygium Aromaticum (clove) and Punica Granatum (Pomegranate) extracts with 2% chlorhexidine in dentinal tubule disinfection against faecalis by using real-time PCR: an in-vitro study.

Abstract

Background: A vital and significant goal in treating a tooth with an apical infection is getting rid of the germs in the pulp space. Recurrent root canal infections are most frequently linked to Enterococcus faecalis and can happen even after endodontic therapy. E. faecalis can resist routine endodontic disinfectants and can also survive the nutrient-deprived conditions in the root filled tooth. So, an attempt has been made to eliminate the microorganisms using the herbal extracts which have antimicrobial properties and comparing it with the well accepted and excellent antimicrobial agent 2% chlorhexidine. Aim: To compare Syzygium aromaticum (Clove) and Punica granatum (Pomegranate) extracts with 2% chlorhexidine in dentinal tubule disinfection with Real-time polymerase chain reaction which was used to detect E. faecalis. Methods: Thirty-six extracted premolar teeth were selected, access cavity was prepared and cleaning and shaping was done. With the help of a rotating diamond disc bur, the middle part of the root was sliced. E. faecalis was applied to the tooth specimens and left on them for 21 days. Group 1 specimens had pomegranate extract, Group 2 contained clove extract, and Group 3 contained 2% CHX. After being watered by the corresponding groups, the specimens were incubated for 5 days. A Gates-Glidden drill was used to collect the dentinal shavings, which were then subjected to DNA isolation before being subjected to real-time PCR analysis. Statistical analysis used: The results were statistically analyzed using one way ANOVA and Post hoc Tukey's analysis. Results: Threshold cycle (Ct) values showed greater inhibition of bacterial load with pomegranate extracts followed by 2% chlorhexidine. Lesser reduction of bacterial load was found with clove extract. Conclusion: Pomegranate extract, an herbal extract with therapeutic potential which can be utilised as an efficient substitute for 2% CHX for treating E. fecalis. Keywords: Clove; Pomegranate; E. Fecalis; Real-time PCR.