Abstract
Pterodon pubescens Benth., popularly known as sucupira, is traditionally used as pain healing agent for many inflammatory diseases. The present study evaluated the in vivo antinociceptive and anti-inflammatory properties of sucupira's fruit dicloromethane extract (Pp) compared to the aqueous extract (Ppa) traditionally used in folk medicine. Extracts' chemical characterization was performed by gas chromatography coupled to mass spectra (GC-MS) detection. The standardized extracts were evaluated using antinociceptive and anti-inflammatory experimental models with mice. The results reported herein allowed establishing a relationship between the popular use of Pterodon pubescens fruit for pain relief and the activity of two major compounds isolated from this species which demonstrated antinociceptive activity. The experimental models corroborate activity of aqueous extract antinociceptive and anti-inflammatory activity, with lower potency compared to dichloromethane extract. Nevertheless the resulting data corroborates sucupira's folk use for pain relief.
Highlights
The study of inflammatory pain has been one of the most rapidly advancing and expanding areas of pain research
The results on the open-field test demonstrated that oral (p.o.) treatment with both dichloromethane P. pubescens fruit extract (Pp) and aqueous P. pubescens fruit extract (Ppa) in a dose-dependent manner based on logarithmic scale (30, 100 and 300 mg kg-1) did not cause any significant changes in the ambulatory behavior of mice
The intraperitoneal (i.p.) treatment with the positive control pentobarbital (35 mg kg-1) significantly (p ≤ 0.001) reduced the locomotor activity of animals on this assay, compared to the group that received vehicle. These data validated the dose-response curves designed for this study, especially for the behavioral methods used for samples antinociceptive activity evaluation, avoiding results misinterpretation
Summary
The study of inflammatory pain has been one of the most rapidly advancing and expanding areas of pain research. The writhing test was carried out as described previously.[27,28] Groups of mice (n = 6) were treated orally (p.o.) with vehicle (10 mL kg-1), dichloromethane P. pubescens fruit extract (Pp) and aqueous P. pubescens fruit extract (Ppa) using three doses 10, 30, and 100 mg kg‐1. Formalin-induced nociception was measured as previously described.[28,29] Groups of mice (n = 6) were treated orally (p.o.) with vehicle (10 mg kg-1), dichloromethane P. pubescens fruit extract (Pp) and aqueous P. pubescens fruit extract (Ppa) using 30, 100, and 300 mg kg-1 doses. The hyper nociceptive response was considered when animals vocalized or withdrew the paw from the equipment, demonstrating pain After this first measurement, animals received a carrageenan (0.1 mL) intraplantar (i.pl.) injection (2.5% in saline) into the right hind paw surface. The data were considered significant at p ≤ 0.05 level
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
