Abstract
ABSTRACTSeveral preparations, aimed as additives to microbial broth media were obtained from waste yeast cells usually discarded by two Bulgarian breweries. The products were assessed with respect to their ability to stimulate microbial growth, simplicity of obtaining, maximal yield of soluble substances from the starting material, necessity of expensive equipment, expenditure of materials, chemicals, and time. It was established that the preparation, obtained by usual autolysis at elevated temperatures, filtration through two sheets of gauze, vacuum concentration, addition of starch, drying, grinding and sieving is the preferable one because of its high ability for stimulating bacterial growth and easiness of obtaining. The turbidity, caused by the starch and some dead yeast cells is no obstacle since they remain blocked in the agar layer. When clear liquid media is necessary to be prepared, these particles are easily eliminated before the sterilisation step of the media by filtration through ordinary filter paper. It is well known that usually, such filtration step is necessary when peptone media are prepared.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
