Abstract
The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were compared four different DNA extraction methods from must and wine samples obtained from eleven V. vinifera varieties (five grapevine varieties for white wines and six grapevine varieties for red wines, respectively). Taking into consideration the amounts of obtained DNA, the concentrations and purities of the final DNA extracts, were stood out two modified methods. For all must samples, very efficient was the second method, which allowed obtaining a mean value of 87.9 ng µl-1 for the DNA concentration with 1.55 purity. Among the tested procedures, for monovarietal wine samples, the fourth method proved to be the most efficient which brought a mean value of 64.7 ng μl-1 for DNA concentration with 1.66 purity. This method adequate for wine samples involves two CTAB solution treatments and the RNase treatment applied before DNA resuspension. The DNA from must and wine extracts and the DNA from leaves of the corresponding grapevine varieties were amplified with five specific microsatellite primers (VVS2, VVMD27, VVMD32, VrZAG79 and VrZAG62). The aspects of pattern profiles were analysed in parallel and proved that the extracted DNA was suitable for amplification with these specific V. vinifera primers. The two selected extraction procedures are considered good for research purposes and ensure obtaining of good-quality extracted DNA from musts and one-year old wines.
Highlights
In countries with tradition of grapevine growing, checking the quality of wines, proving their origin and authenticity before marketing are priority concerns
For the selection of the most efficient methods applied in DNA extraction from must and wine samples, the concentration of the final extract and its quality are major criteria
Our results with four different methods showed that an amount of 45 ml of must or wine and an initial precipitation with sodium chloride ensured a final extract with a good concentration of the residual DNA and in a sufficient amount for further investigations
Summary
In countries with tradition of grapevine growing, checking the quality of wines, proving their origin and authenticity before marketing are priority concerns. In accordance with Directive 2000/13/EC and up-to-date national legislation, all stages of the agri-food supply chain must be monitored through a traceability system. The quality of any food product first of all depends on its origin and the accurate identification of the variety / varieties from which that product was obtained (Siret et al, 2000). Reliable methods of traceability along the entire chain, from the vineyard plant to the wine in the bottle, have been improved during the last 20 years on Received: 22 Apr 2021. From Volume 49, Issue 1, 2021, Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal uses article numbers in place of the traditional method of continuous pagination through the volume. The journal will continue to appear quarterly, as before, with four annual numbers
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