Comparison of short term in vitro cultured human mast cells from different progenitors — Peripheral blood-derived progenitors generate highly mature and functional mast cells

Abstract

During the last two decades different scientific groups have investigated the phenotype and function of in vitro generated human mast cells (MC). The cells have been shown to display variable surface markers and functional characteristics. The phenotypic differences may reflect different culture conditions, protocols or the use of different progenitors.To investigate the significance of different progenitors, we have compared MC generated from CD133+ progenitor cells from cord blood (CBMC) or peripheral blood (PBMC). The progenitors were cultured for 7 weeks in the presence of IL-6 and SCF, with addition of IL-3 the first 3 weeks, and FCS during week 7. The phenotype of the established MC was characterized by surface marker expression levels, metachromasia, histamine and tryptase contents and their function was evaluated by receptor-mediated release of histamine and PGD2.The generated metachromatic (<99%) MC were 75% tryptase+, regardless of the source of progenitor cell. Expression of c-kit/CD117, CD203c, and FcεRI was comparable. The density of c-kit/CD117 receptors on CBMC was higher that of PBMC (p<0.001). The density of CD203c and FcεRI was higher on PBMC (p<0.001). PBMC contained more histamine (p<0.001), expressed more FcεRI (p<0.001) and released more histamine (p<0.001) and PGD2 (p<0.001) upon ligation of FcεRI, than CBMC.Culture with IL-4 increased expression of tryptase, FcεRI, CD117 and CD203c, secretion of histamine and PGD2 of PBMC, and histamine secretion of CBMC.Cord and peripheral blood may give rise to different types of MC. The question addressed should determine the progenitor cell and protocol to be used.