Abstract

AKR MuLV 70S RNA was separated on Poly(U)-Sepharose into poly(A) and non-poly(A) containing 34S subunits. The ratio of the two fractions was 2:1, respectively. Both fractions were hybridized to AKR MuLV [ 3H]cDNA, and the hybrids were assayed by nuclease S 1 and cesium sulfate centrifugation. The poly(A) and non-poly(A) subunits hybridized to [ 3H]cDNA to the same extent (80%), with identical CO 1 2 values; and the hybrids of both fractions had identical T m values (81°C in 0.15 M NaCl). These results demonstrate that the poly(A) and non-poly(A) containing subunits of the AKR genome have identical or very similar base sequences in the heteropolymeric regions.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call