Abstract
Relative retention analysis and increment approach were applied for the comparison of triglycerides (TGs) retention of a broad set of plant seed oils with isomeric conjugated octadecatrienoic acids (CLnA) by reversed-phase HPLC for “propanol-2-acetonitrile” mobile phases and Kromasil 100-5C18 stationary phase with diode array detection (DAD) and mass spectrometric (MS) detection. The subjects of investigation were TGs of seed oils: Calendula officinalis, Catalpa ovata, Jacaranda mimosifolia, Centranthus ruber, Momordica charantia, Trichosanthes anguina, Punica granatum, Thladiantha dubia, Valeriana officinalis, and Vernicia montana. It was found that a sequence of elution of TGs of the same types is the same without any inversions for full range of mobile phase compositions: punicic (C18:39Z11E13Z) < jacaric (C18:38Z10E12Z) < catalpic (C18:39E11E13Z) < α-eleostearic (C18:39Z11E13E) < calendic (C18:38E10E12Z) < β-eleostearic (C18:39E11E13E) < all-E calendic (C18:38E10E12E) acids. TGs and fatty acid compositions were calculated for all oil samples. Regularities of solute retentions as a function of isomeric conjugated octadecatrienoic acid moiety structure are discussed. Thus, it was proven that it is possible to differentiate TGs of complex composition with moieties of all natural CLnA by retention control accomplished by electronic spectra comparison, even though there are only three types of electronic-vibration spectra for seven isomeric CLnA.
Highlights
Among a great variety of plant seed oils, oils with conjugated fatty acid moieties are of special interest because of their high biological activity [1,2,3,4,5,6,7]
Oils were extracted for plant seeds: Calendula officinalis, Catalpa ovata, Momordica charantia and Punica granatum grown in Belgorod; Thladiantha dubia and Valeriana officinalis, bought in Belgorod market for gardeners; and Trichosanthes anguina, Jacaranda mimosifolia and Vernicia montana, grown in Vietnam in 2016
Restricted number of the natural isomers was found in plant sources
Summary
Among a great variety of plant seed oils, oils with conjugated fatty acid moieties are of special interest because of their high biological activity [1,2,3,4,5,6,7]. The first way implies transmethylation procedure of initial oils to obtain methyl esters of the fatty acids suitable for subsequent analysis by gas chromatography [10]. This method permits determination the oil main fatty acids as well as minor components. It should be Separations 2017, 4, 37; doi:10.3390/separations4040037 www.mdpi.com/journal/separations
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