Comparison of Semen Diluted Basis (Tris-Egg Yolk) with Diluted Commercial Semen (Andromed®) in Frozen Goat Semen on Sperm Quality by CASA

Abstract

The process of producing frozen goat semen involves a preparation of semen diluents, which is complicated. Since it involves a rapid temperature reduction, the quality of the sperm deteriorates. This study aimed to determine the comparison of semen diluted basis (Tris-egg yolk) with diluted commercial semen (Andromed®) in frozen goat semen on sperm quality by Computer aided sperm analysis (CASA) after thawing at 0, 1 and 3h. by using 1 Goat (Boer) semen. The semen was collected by means of an artificial vagina diluted with both types of semen diluent. Then the semen was preserved by a freezing method. The results showed that the preliminary assessment of fresh semen quality was good and suitable for use in frozen semen production. Additionally, it was found that frozen goat semen diluted basis (Tris-egg yolk) and diluted commercial semen (Andromed®) had no significant difference in motility, progressive motility, VAP, VSL, VCL, ALH, BCF (HZ), STR (%) and LIN (%) at 0h. However, at 1h, the motility and the progressive motility of Andromed® were 87.20±2.71% and 46.98±6.03%, while those of Tris-egg yolk were 68.55±11.5% and 34.20±4.42% respectively which these values were significantly different (P<0.05). At 3h, the motility and the progressive motility of Andromed® were 73.45±3.04% and 31.58±6.63%, while those of Tris-egg yolk were 10.43±1.63% and 1.37±0.06% respectively which these values were significantly different (P<0.01). In addition, at 1 and 3h, the VAP, VSL, VCL, ALH, and STR values of Andromed® diluted sperm were higher than those of Tris-egg yolk. From this study, it was found that commercial semen diluent (Andromed®) helps to maintain the quality of frozen goat semen and reduce the steps of semen diluent preparation. It can also be applied in farms or agencies.