Comparison of sealing ability of mineral trioxide aggregate, biodentine with and without bioactive glass as furcation repair materials: An ultraviolet spectrophotometric analysis

Abstract

Aim: The aim of this study was to evaluate the sealing ability of mineral trioxide aggregate (MTA), Biodentine with and without Bioactive glass (BG) as furcation repair materials by ultraviolet (UV) spectrophotometric analysis. Materials and Methods: Forty extracted human maxillary molars (n = 40) were selected and decoronated 3 mm above the cementoenamel junction and 3 mm below it and a defect in furcation was created. The samples were then divided into 4 groups of 10 (n = 10) each, Group I: MTA, Group II: Biodentine, Group III: BG + Biodentine and Group IV: BG + MTA and the defect was treated with respective furcation repair material. All the samples were then immersed in 2% methylene blue solution for 24 h and later stored in 65% nitric acid solution. The solution obtained was subjected to centrifuge at 3500 rpm for 5 min. From this solution, 100 μl of the supernatant was collected, analyzed in UV spectrophotometer at 550 nm with nitric acid as the blank and readings were recorded as absorbance units. Results: All four groups exhibited a significant difference in dye absorbance values (P < 0.01). Group I, i.e., MTA showed the least dye absorbance values when compared with the other three groups. Data were analyzed using one-way analysis of variance and post hoc Tukey tests. The level of statistical significance was set at P < 0.05. Conclusion: Within the limitations of the study, it can be concluded that MTA had superior sealing ability than Biodentine, whereas BG + Biodentine showed better sealing ability when compared with BG + MTA.