Comparison of salivary levels of mucin and amylase and their relation with clinical parameters obtained from patients with aggressive and chronic periodontal disease

Andrea Beatriz Acquier,Lucila Busch,Gabriel Antonio Sánchez,Alejandra Karina De Couto Pita

doi:10.1590/1678-775720140458

Abstract

Objective Salivary mucin and amylase levels are increased in patients with chronic periodontitis (CP). Due to the fact that aggressive periodontitis (AgP) not only differs from chronic periodontitis in terms of its clinical manifestation, the aim of this study was to compare salivary mucin and amylase levels and their relation to the clinical parameters of patients with aggressive periodontitis with that of patients with chronic periodontitis.Material and Methods Eighty subjects were divided into two groups: 20 patients with AgP and their 20 matched controls and 20 patients with CP and their 20 matched controls, based on clinical attachment loss (CAL), probing pocket depth (PPD) and bleeding on probing (BOP). Whole unstimulated saliva was obtained and mucin, amylase and protein were determined by colorimetric methods. Pearson’s correlation analysis was used to determine the relationship between salivary mucin, amylase and protein levels and the clinical parameters.Results Salivary mucin, amylase and protein levels were increased in patients with AgP and CP but there were no differences between them or between control groups. Pearson’s correlation analysis, determined in the entire subjects studied, showed a positive and significant correlation of mucin, amylase and proteins with CAL and PPD and a negative correlation with the flow rate. When Pearson’s correlation analysis was carried out in each group separately, Fisher’s z transformation showed no significant difference between both groups.Conclusion Comparison of the salivary levels of mucin, amylase and protein and their relationship with clinical parameters of AgP patients with that of CP patients revealed no differences between both groups.

Highlights

Chronic and aggressive periodontitis consists of an inflammatory reaction of the periodontal tissues in response to infection caused by a specific group of bacteria
Since there was a significant difference in the age between Aggressive periodontitis (AgP) and chronic periodontitis (CP) patients, two control groups were formed with subjects of similar ethnicity, income levels, age and gender to patients
As can be seen in the table, both disease groups showed significantly higher clinical attachment loss (CAL) and probing pocket depth (PPD) but lower unstimulated flow rate than the control groups, when tested by one-way ANOVA followed by Neuman-Keuls multiple comparison

Summary

Introduction

Chronic and aggressive periodontitis consists of an inflammatory reaction of the periodontal tissues in response to infection caused by a specific group of bacteria. Aggressive periodontitis (AgP) is characterized by severe and rapid loss of periodontal attachment, often commencing at or after the pubertyChronic periodontitis (CP) is a common disease that is prevalent among adults and seniors. The aggressive nature of AgP depends on the bacterial etiology, host susceptibility, hereditary and environmental factors, and often behavioral factors. There are numerous defense proteins present in saliva. Some of these defense proteins, such as salivary immunoglobulins, and salivary chaperokine HSP70/HSPA, are involved in both innate and acquired immune activation. Salivary cationic peptides and other salivary defense proteins, such as lysozyme, BPI, BPI-like and PLUNC proteins, salivary amylase, cystatins, prolin-rich proteins,

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Results

Conclusion