Comparison of saccharification process by acid and microwave-assisted acid pretreated swine manure

Abstract

The saccharification process of swine manure by conventional and microwave-assisted acid pretreated were investigated using cellulose enzymes, respectively. The optima for microwave-assisted acid pretreated swine manure is achieved when swine manure of 50 g l(-1) of substrate concentration and water amount 40 ml was pretreated by 4% H2SO4 concentration with 445 W microwave powers for 30 min at pretreatment period, and temperature 50 degrees C, enzyme loading 2 mg g(-1) substrate, substrate concentration 5 g l(-1) and initial medium pH 4.8 at enzymes hydrolysis period by microwave-assisted acid pretreated, respectively. The optimal conditions by conventional acid pretreated is obtained when 50 g l(-1) swine manure was submerged in 40 ml, 4% H2SO4 maintained at 130 degrees C for 3 h at pretreatment period, and temperature 45 degrees C, enzyme loading 2 mg g(-1) substrate, substrate concentration 15 g l(-1) and initial medium pH 5.2 at enzymes hydrolysis period, respectively. Under the optimum conditions microwave-assisted acid pretreatment could achieve higher yield of reducing sugar, short reaction time, and lower energy consumption than from the conventional acid pretreatment, which indicates that microwave-assisted acid pretreatment is more suitable for swine manure pretreatment than by acid alone.