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Abstract
Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples
Highlights
Brucellosis is one of the most common neglected zoonotic diseases, and it has continued to widely affect the world’s population with up to 500,000 new cases worldwide annually in humans, greatly affecting the developing countries such as India, Uganda, Sudan, and among others [1,2,3]
It appears that rose bengal plate test (RBPT) is good as a screening test, whereas serum agglutination test (SAT) and indirect enzyme-linked immunosorbent assay (i-enzyme-linked immunosorbent assay (ELISA)) are good for diagnosis purposes or confirmatory test
An inter-rater reliability test was performed to analyze the degree of agreement between RBPT, SAT, and i-ELISA using Fleiss’ kappa
Summary
Brucellosis is one of the most common neglected zoonotic diseases, and it has continued to widely affect the world’s population with up to 500,000 new cases worldwide annually in humans, greatly affecting the developing countries such as India, Uganda, Sudan, and among others [1,2,3]. Confidence interval [CI]: 3.6-10.0%) and the adjusted individual animal prevalence was 5.0% (21/423, 95% CI: 2.7-9.3%) based on diagnosis using commercial kits of the competitive enzyme-linked immunosorbent assay (ELISA) for Brucella abortus antibodies [4]. Several methods are used to detect and screen brucellosis that includes rose bengal plate test (RBPT), ELISA, serum agglutination test (SAT), complement fixation test, indirect hemolysis test, and among others [5]. In Uganda, detection of brucellosis in goats and humans is more frequently done using rose bengal plate test (RBPT) in comparison to other serological tests such as serum agglutination test (SAT) and indirect enzyme-linked immunosorbent assay (i-ELISA)
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