Comparison of Results of Semen Parameters Prepared by Swim-up Technique Between the Effect of Adding Autologous Platelet-Rich Plasma and Phosphate Buffer Saline to the Human Semen

Abstract

The most popular technique of making ready sperm is the swim-up procedure, in which sperm is selected on motility and ability to swim out the plasma of the semen due to the plasma contains decapacitation factors that alter sperm's ability to fertilize. This research covers 119 semen samples from men visiting the fertility clinic of the High Institute for Infertility Diagnosis and ART that included normal and abnormal sperms parameters as stated in the WHO. The semen sample was dissected into 2 portions: First grouping was affected by the equal quantity of phosphate buffer saline (250) μm contain 10% human serum albumin (HSA) while the second grouping was affected by the equal quantity of phosphate buffer saline (250) μm contain 10% human serum albumin (HSA) with added 2% platelet-rich plasma. Then samples are prepared by the swim-up method for evaluation parameters of the sperm. Swim-up PBS+PRP(HAS) media resulted in the highest sperm concentration, highest total count, highest total motility, highest progressive motility A+B, A, and B, the highest concentration of active motile sperm % and highest normal morphology sperm % after that swim up –PBS (HAS), sequentially in a highly significant manner (p < 0.001). We conclude the insertion of PRP resulted in results that are technically far two methods with regarding sperm concentration, total count, total motility, progressive motility A+B, A, and B, non-progressive motility (C), immotile sperm (D), the concentration of active motile sperm % and normal morphology sperm and the values were far better in Swim-up PBS (HAS) with added 2% PRP than those produced by swim-up –PBS (HAS) alone