Abstract

Redigel, a unique pectin-based media system, was compared with various conventional media using American Type Culture Collection organisms. The system consists of a liquid inoculating medium for isolation and identification and a calcium-coated plastic Petri dish. When the pectin medium is poured into the Petri dish, the calcium causes the pectin to harden. Results with the Redigel pectin media showed that the various gram-negative, grampositive, and yeast cultures all gave acceptable reactions when compared with conventional media. The Mueller-Hinton medium, however, is not recommended for use in the disk diffusion test when susceptibility data for Pseudomonas aeruginosa against aminoglycosides is desired. The calcium ions used for solidification will interfere with the zone sizes.

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