Abstract
BackgroundRadix Polygoni Multiflori is the dried root tuber of Polygonum multiflorum Thunb. (Fam. Polygonaceae). According to Chinese medicine theory, raw (R-RPM) and processed (P-RPM) Radix Polygoni Multiflori possess different properties. The present study investigates the differences in chemistry between raw and processed Radix Polygoni Multiflori.MethodsFive pairs of R-RPM and P-RPM as well as 15 commercial decoction pieces were analyzed with high performance liquid chromatography (HPLC) and mass spectrometry (MS).ResultsTwo anthraquinones, namely emodin-8-O-(6'-O-malonyl)-glucoside and physcion-8-O-(6'-O-malonyl)-glucoside disappeared or decreased significantly and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside, emodin-8-O-β-D-glucopyranoside and physcion-8-O-β-D-glucopyranoside decreased after the R-RPM samples being processed. On the other hand, the contents of emodin and physcion generally increased after processing.ConclusionThe present study indicates that processing Radix Polygoni Multiflori may change the contents and types of chemicals in it. These changes are probably responsible for the various pharmacological effects of R-RPM and P-RPM as well as hepatotoxicity.
Highlights
Radix Polygoni Multiflori is the dried root tuber of Polygonum multiflorum Thunb
Optimization and validation of high performance liquid chromatography (HPLC) conditions To optimize the elution conditions, we investigated the mobile phase of acetonitrile-water with various gradients and the optimal acetonitrile-water system was determined to have acetonitrile adjusted to 10%, 35%, and 100%, at 0, 45 and 65 min, respectively
The present study demonstrates that processing Radix Polygoni Multiflori may change the contents, the quantity and types of chemicals in it
Summary
Radix Polygoni Multiflori is the dried root tuber of Polygonum multiflorum Thunb. According to Chinese medicine theory, raw (R-RPM) and processed (P-RPM) Radix Polygoni Multiflori possess different properties. The present study investigates the differences in chemistry between raw and processed Radix Polygoni Multiflori. Proper pharmaceutical processing may reduce toxicity or side effects, potentiate the beneficial effects, change the pharmacological properties, preserve active constituents, facilitate administration, improve flavor or correct unpleasant taste and increase purity of Chinese materia medica [1,2,3,4]. Radix Polygoni Multiflori (Heshouwu) is the dried root tuber of Polygonum multiflorum Thunb. According to Chinese medicine theory, raw Radix Polygoni Multiflori (R-RPM) counteracts toxicity, cures carbuncles and relaxes the bowels whereas processed Radix Polygoni Multiflori (P-RPM) replenishes the liver and kidney with. R-RPM inhibited triglyceride accumulation induced by carbon tetrachloride (CCl4), cortisone acetate and thioacetamide (TAA) in the mouse liver and P-RPM lowered the triglyceride accumulation induced by cortisone acetate; both R-RPM and P-RPM reduced liver enlargement caused by CCl4 [9]
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