Abstract
Method Fifty isolates of MRSA from different specimens like blood, urine, exudates and respiratory were collected from 2010 to 2011. MRSA screen slide latex agglutination test kit (Denka Seikan, Japan) was used for PBP2′ detection and Oxacillin Resistant Screen Agar Base (ORSAB) with supplement (Oxoid Ltd., UK) was used for detection of MRSA from the isolates with ATCC controls. The detection of mecA gene in the MRSA isolates was considered as the reference method for determining the sensitivity of each phenotype rapid method studied.
Highlights
Detection of methicillinresistant Staphylococcus aureus (MRSA) in clinical specimens is imperative to prevent cross transmission, morbidity, mortality and overall cost of treatment
rapid detection of MRSA helps in preventing superficial skin infection to become deep seated
The detection of mecA gene in the MRSA isolates was considered as the reference method for determining the sensitivity
Summary
Comparison of rapid methods PBP2′ detection, ORSAB and mecA for detection of methicillinresistant Staphylococcus aureus (MRSA) in a tertiary care centre, Chennai, India. From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014) Chennai, India. 30 January - 1 February 2014
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