Abstract
The efficacy of candidate DNA vaccines based on the smallpox natural gene A30L and artificial gene A30Lopt with modified codon composition optimized for expression in mammalian cells was tested. Groups of mice were intracutaneously immunized three times at 3-week intervals with candidate DNA vaccines, i.e., pcDNA_A30L or pcDNA_A30Lopt. Three weeks after the last immunization, all groups of mice were intraperitoneally infected by the K1 strain of ectromelia virus in a dose of 10 LD50 to estimate protection. It was shown that the immunization of mice by DNA vaccines based on natural gene A30L and artificial gene A30Lopt with optimized codon composition caused the production of virus-neutralizing antibodies and provides protection from a lethal dose of ectromelia virus with an insignificant advantage for the A30Lopt gene.
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