Comparison of Properties of Stem Cells Isolated from Adipose Tissue and Lipomas in Dogs.

Takahiro Teshima,Maika Shiba,Kazuho Dairaku,Ryohei Suzuki,Hirotaka Matsumoto,Hidekazu Koyama,Akito Matsuoka

doi:10.1155/2019/1609876

Takahiro Teshima, Maika Shiba + Show 5 more

Open Access

https://doi.org/10.1155/2019/1609876

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Abstract

Adipose-derived mesenchymal stem cells (ADSCs) have been suggested their benefits in regenerative medicine for various diseases. Lipomas, benign neoplasms in adipose tissue, have been reported as a potential source of stem cells. These lipoma-derived mesenchymal stem cells (LDSCs) may be useful for regenerative medicine. However, the detailed characteristics of LDSCs have not been fully elucidated. This study investigated the cellular proteomics and secretomes of canine LDSCs in addition to morphology and proliferation and differentiation capacities. Some LDSCs isolated from canine subcutaneous lipomas were morphologically different from ADSCs and showed a rounded shape instead of fibroblast-like morphology. The phenotype of cell surface markers in LDSCs was similar to those in ADSCs, but CD29 and CD90 stem cell markers were more highly expressed compared with those of ADSCs. LDSCs had noticeably high proliferation ability, but no significant differences were observed compared with ADSCs. In regard to differentiation capacity compared to ADSCs, LDSCs showed higher adipogenesis, but no differences were observed with osteogenesis. Cellular proteomic analysis using two-dimensional gel electrophoresis revealed that over 95% of protein spots showed similar expression levels between LDSCs and ADSCs. Secretome analysis was performed using iTRAQ and quantitative cytokine arrays. Over 1900 proteins were detected in conditioned medium (CM) of LDSCs and ADSCs, and 94.0% of detected proteins showed similar expression levels between CM of both cell types. Results from cytokine arrays including 20 cytokines showed no significant differences between CM of LDSCs and that of ADSCs. Our results indicate that canine LDSCs had variability in characteristics among individuals in contrast with those of ADSCs. Cellular proteomics and secretomes were similar in both LDSCs and ADSCs. These findings suggest that LDSCs may be suitable for application in regenerative medicine.

Highlights

Mesenchymal stem cells (MSCs) are adult multipotential progenitors with demonstrated important utility in regenerative medicine
Most Lipomaderived MSCs (LDSCs) were similar in shape to Adipose tissue-derived MSCs (ADSCs), but cells in some individuals showed a rounded shape unlike ADSCs (Figure 1)
A higher expression of CD29 was noticed in LDSCs at passages 5 and 6 (P < 0:05) compared with that of ADSCs

Summary

Introduction

Mesenchymal stem cells (MSCs) are adult multipotential progenitors with demonstrated important utility in regenerative medicine. Several studies reported the properties of LDSCs [5,6,7,8,9,10], and most reports investigated cell surface markers, proliferation, and multilineage differentiation including adipogenesis, osteogenesis, and chondrogenesis, with only functional research on anti-inflammatory effects [9] These studies suggested that LDSCs were a good source of MSCs and might be as useful as ADSCs. One advantage of ADSCs is that adipose tissues can be obtained with minimal invasive procedures such as liposuction aspirates or adipose tissue biopsies, but it is necessary for invasion even though minimal.

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