Abstract
We have used ultrafast spectroscopy to compare the photochemistry of Photosystem II (PS II) reaction centres isolated from the higher plant pea, Pisum sativum, and a green alga, Chlamydomonas reinhardtii. The apparatus now used for these experiments includes a highly sensitive multichannel detector and a magic angle configuration for the pump and probe beam polarisations. For both organisms, identical time constants were obtained for both the formation of the radical pair P680 +Ph −, and charge recombination from this state. We conclude that the use of the organism Chlamydomonas reinhardtii is well suited to future studies of the primary photochemistry of genetically mutated PS II reaction centres.
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