Abstract
This study was carried out to see if there are any differences between the prevalence of Kaposi’s sarcoma in HIVinfected patients with antibody to Human Herpes Virus 8 (HHV8) or not.Methods: The sera of 180 subjects were evaluated for HIV and HHV8 using double ELISA (Genscreen UltraHIV Ag-Ab ELISA and JN HIV 1/2 ELISA Kit) for HIV screening and advanced Biotechnology IncorporatedELISA for HHV8 IgG antibody detection respectively. Those HIV infected patients that had skin lesionssuspected to be Kaposi’s sarcoma were biopsied for histological diagnosis.Results: A total of 180 subjects were recorded for this study. The ratio of male: female was 1:1.02. In all, 100subjects tested positive to HIV screening and 87 (87%) patients tested positive to HHV8 antibody. Of the 87patients that were positive to HHV8 antibodies 32 (36.7%) were histologically confirmed to have Kaposi’ssarcoma.Conclusion: We found a high rate of prevalence of Kaposi’s sarcoma KS among HIV infected patients withHHV8 antibodies.
Highlights
This study was carried out to see if there are any differences between the prevalence of Kaposi’s sarcoma in HIV infected patients with antibody to Human Herpes Virus 8 (HHV8) or not
The sera of 180 subjects were evaluated for HIV and HHV8 using double enzyme linked immunosorbent assay (ELISA) (Genscreen Ultra HIV Ag-Ab ELISA and JN HIV 1/2 ELISA Kit) for HIV screening and advanced Biotechnology Incorporated ELISA for HHV8 IgG antibody detection respectively
We found a high rate of prevalence of Kaposi’s sarcoma KS among HIV infected patients with HHV8 antibodies
Summary
This study was carried out to see if there are any differences between the prevalence of Kaposi’s sarcoma in HIV infected patients with antibody to Human Herpes Virus 8 (HHV8) or not. Methods: The sera of 180 subjects were evaluated for HIV and HHV8 using double ELISA (Genscreen Ultra HIV Ag-Ab ELISA and JN HIV 1/2 ELISA Kit) for HIV screening and advanced Biotechnology Incorporated ELISA for HHV8 IgG antibody detection respectively Those HIV infected patients that had skin lesions suspected to be Kaposi’s sarcoma were biopsied for histological diagnosis. HIV screening and confirmation was done based on two positive results of screening test using two different methods namely: dip stick immunocoomb and conventional enzyme linked immunosorbent assay (ELISA) according to standard and manufacturer’s instructions Their sera were further tested for HHV-8 IgG antibodies using whole extract virus lysate from Advanced Biotechnology Incorporated, U.S.A. An elliptical incisional skin biopsy was done for every skin lesion suspected to be Kaposi’s sarcoma in HIV infected patients. Each of the slides was viewed under light microscope at different magnifications ranging from X40 to X100 to confirm Kaposi’s sarcoma histologically
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