Abstract
Ganoderma lucidum and Ganoderma sinense, known as Lingzhi in Chinese, are commonly used Chinese medicines with excellent beneficial health effects. Triterpenes and polysaccharides are usually considered as their main active components. However, the content of triterpenes differs significantly between the two species of Ganoderma. To date, a careful comparison of polysaccharides from the two species of Ganoderma has not been performed. In this study, polysaccharides from fruiting bodies of two species of Lingzhi collected from different regions of China were analyzed and compared based on HPSEC-ELSD and HPSEC-MALLS-RI analyses, as well as enzymatic digestion and HPTLC of acid hydrolysates. The results indicated that both the HPSEC-ELSD profiles and the molecular weights of the polysaccharides were similar. Enzymatic digestion showed that polyshaccharides from all samples of Lingzhi could be hydrolyzed by pectinase and dextranase. HPTLC profiles of their TFA hydrolysates colored with different reagents and their monosaccharides composition were also similar.
Highlights
Ganoderma, known as “Lingzhi” in Chinese, is a genus of fungi belonging to the familyPolyporaceae
The results indicated that polysaccharides from G. lucidum and G. sinense were similar, based on the retention time
Coupled with multi-angle laser light scattering (MALLS) and refractive index (RI) detection, which showed that the molecular weights (Mw) values of peak I and peak II in GLP and GSP were 4.35 × 106 (±0.7%) and
Summary
Known as “Lingzhi” in Chinese, is a genus of fungi belonging to the family. 98 species of Ganoderma have been found in China. Only two species of Ganoderma, G. lucidum and G. sinense, are recorded in the Chinese Pharmacopoeia (2010). Triterpenes and polysaccharides are usually considered the main active components in Lingzhi, the content of triterpenes differs significantly between the two species of Ganoderma [1]. Modern pharmacologic studies have revealed that polysaccharides have multiple pharmacological activities [2,3,4]. High-performance liquid chromatography (HPLC), gas chromatography (GC), mass spectrometry (MS), nuclear magnetic resonance (NMR) [9,10] and high-performance thin-layer chromatography (HPTLC) [11,12] have been used for determination of chemical properties of polysaccharides from Lingzhi. Polysaccharides from the two species of Lingzhi collected from different regions of China were analyzed and compared. It is helpful to elucidate the characters of polysaccharides from Lingzhi, which is beneficial for quality control
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