Abstract
To compare platelet rich plasma (PRP) and fibrin glue about the effect of anastomotic healing. Thirty six Wistar-Albino male rats diveded into 3 groups according to control(Group1), PRP (Group 2) and fibrin glue(Tisseel VH) (Group 3). The colon was transected with scissor and subsequently an end to end anastomosis was performed using continuous one layer 6/0 vicryl sutures. Postoperative 7th day effect of anastomotic healing measuring with tissue hydroxyproline(TH) level and anastomotic bursting pressure(ABP); moreover comparison of cytokine (IL-6 and IL-10) and procalcitonin levels on 1st,3rd and 7th days. There was no statistically significant difference of the ABP and hydroxyproline levels between PRP and fibrin glue on the 7th day. There was no statistically significant difference between levels of proinflammatory cytokine (IL-6) (P=0.41), anti-inflammatory cytokine (IL-10) (P=0.35), and procalcitonin levels (P=0.63) on 1, 3 and 7 days. Fibrin glue and platelet rich plasma are shown to be effective in healing intestinal anastomoses without superior to each other.
Highlights
To compare platelet rich plasma (PRP) and fibrin glue about the effect of anastomotic healing
PRP (Platelet-rich plasma) and fibrin glue are frequently used in studies because they are accessible in clinical practice, simple to administer and available everywhere
The anastomosis was resected with a margin of at least 4 cm on each side with regard to tissue hydroxyproline level followed by measuring the anastomotic bursting pressure (ABP)
Summary
This study was established at the Experimental Research Center after obtaining the ethical committee approval of Cukurova University, Faculty of Medicine (Approval number: 2016/12). Thirty six Wistar-Albino male rats, weighting 250–300 g were used in the present study. The animals were maintained at 2°C, humidity at 40–60% with a 12 hr light/dark cycle and allowed free access to water and standard chow during the study. All animals were observed closely and weighed on days 7 after surgery. This research was carried out in accordance with the Guide for the Care and Use of Laboratory Animals. The colon was transected with scissor and subsequently an end to end anastomosis was performed using continuous one layer 6/0 vicryl sutures (Figure 1). In the post-operative period, no antibiotics were given to the rats. Oral food was started on the post-operative first day. On postoperative day 7 all animals were euthanized by intraperitoneal overdose of 2 ml pentobarbital sodium (175mg/ml, KU Life, Copenhagen, Denmark). The rat fascia was opened and the anastomosis was achieved by carefully dissecting the adhesions
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