Abstract
THE discovery of deoxyribonucleic acid (DNA) in mitochondria1–9 has opened the way for investigations of the long-standing problem of extra-genome cytogenetic determinants. DNA fibres have been found in mitochondria from tissues belonging to various different classes of animals1. The principal difficulty to be dealt with in the isolation of mitochondrial DNA is caused by the necessity of separating the soluble nuclear DNA, adsorbed on the surface of mitochondria. For this purpose we used a method of purification of mitochondria in ‘Urographin’ density gradient8 or treatment of the mitochondrial fraction with DNase (150 µg/ml. for 1.5 h at 0° C). Fibres of high-polymer deproteinized DNA were obtained by means of the phenol method after Kirby10 and Marmur11. The average DNA content in liver mitochondria amounted to 0.65 µg/mg protein.
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