Abstract
Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata). The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage. Moreover, inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity were investigated. Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains. Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher. These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity. This research provides a guide for the selective application of Massa Medicata Fermentata.
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