Abstract
To days, The Growth hormone (GH) gene is more important in the regulator of metabolism, osmoregulation, reproduction and skeletal growth in Livestock. GH almost in all of animals has been same function that mentioned. This hormone also is exciting skeletal cellular for more growth and replication. In Salmonids for specially, furthermore, GH gene in population of salmonids can be used as the studies of phylogenetics and finding ancient and pedigree of salmonids that some researchers used from GH gene in salmo salar and salmo trutta for studies of phylogenetics. In this study we had done sequence of fragments of GH gene in salmo trutta caspius full length of almost 2048 bp. and deposited in GeneBank (accession number, JN24163) For sequencing of GH gene in the Salmo trutta caspius, first was extracted DNA genomics from bloods and the muscles of salmons, in related to, we designed three pairs of primers from first to end of the GH gene in same sequences from salmo salar and salmo trutta that reported in GeneBank. After sequencing of fragments we analyzed fragments and compared with other sequences in salmonid fishes. In this research our aims, study of amount variation in the between salmo trutta caspius species with Atlantic salmon and also, study amount of phylogenetic variation between Salmo trutta caspius with other salmons regarding to the GH gene.
Highlights
Growth hormone (GH) gene has been very benefits in natural salmonids
According to the annotation GH genes, these are genes linked to economic traits and polymorphism genetics which are governed by many genes, following to the sequences of the salmon GH gene were published in the BLASTn on the National Centre for Biotechnology Information (NCBI) Network Service, was designed a fragment of almost 3kb
There are some genes that finding ancestral of salmonids, these genes including, mitochondrial genomics that more inherited of maternal traits (Allendorf et al, 1984; Bernatchez et al, 1992; Bernatchez, 1995), and GH gene that inherited of paternal traits (Gross and Nilsson, 1995)
Summary
Growth hormone (GH) gene has been very benefits in natural salmonids. The growth hormone receptor on target cells by transducting the myogenic stimulating signal across the cell membrane and inducing the transcription of many genes, including IGF-I (Rotwein et al, 1994).The GH gene effective on the cell growth rate, and, it has been other function for polymorphism of populations in animals. The many genes were used for polymorphism of salmonids, mitochondrial DNA has been studied extensively in numerous fish species by restriction site analysis of the entire mitochondrial genome or by 'micro restriction' mapping and direct sequencing of the selectively amplified genes (Beckenbach, 1991; Bernatchez et al, 1992; Whitmore et al, 1992; Ovenden et al, 1993), that used some genes for this means, including, cytochrome b gene in salmo treutta fario (Rezaei and Akhshabi, 2012), cytochrome b in salmo trutta caspius (Jamshidi and Kalbasi, 2009), and other genes in mitochondrial DNA.
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