Abstract
Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the most economically important diseases in pigs, worldwide. Just in the US, the total costs to the swine industry have been estimated at $664 million per year. Therefore, the continuous and reliable monitoring of the PRRS status of a pig herd is required in order to prevent and reduce costs due to this infection. Mostly used methods for diagnosis of PRRS infection nowadays are serological (ELISA) and molecular (PCR) ones. This study aimed to assess the sensitivity and specificity of three different commercially available ELISA kits for detection of antibodies against PRRSV (IDEXX PRRS X3 Ab Test (IDEXX, USA), INgezim PRRS Universal (Ingenasa, Spain), Pigtype PRRSV Ab (Qiagen, Germany)) using 91 blood serum samples collected from pigs in Serbia. Our study showed no significant differences in specificity and sensitivity between three commercially available ELISA kits. However, IDEXX ELISA proved to be more reliable kit for detecting antibodies against PRRSV with sensitivity of 97,4% and specificity of 98,1%, considering INgezim and Qiagen kits specificity of 92,5% and 83%, respectively, and sensitivity of 94,7 % for both kits. In order to achieve maximal reliability of obtained results, ELISA diagnostic protocol for diagnosis of PRRS infection should be complemented with additional tests such as PCR and virus neutralization test.
Highlights
Porcine reproductive and respiratory syndrome (PRRS) is a contagious viral infection and one of the most common infectious diseases of swine globally, responsible for significant economic losses worldwide
This study aims to assess the sensitivity and specificity of three different commercially available enzyme-linked immunosorbent assay (ELISA) kits for detection of antibodies against PRRSV (IDEXX Porcine Reproductive and Respiratory Syndrome (PRRS) X3 Ab Test (IDEXX, USA), INgezim PRRS Universal (Ingenasa, Spain), and Pigtype PRRSV Ab (Qiagen, Germany)) using 91 blood serum samples collected from pigs in Serbia
Our study showed a certain level of differences in specificity and sensitivity between three commercially available ELISA kits
Summary
Porcine reproductive and respiratory syndrome (PRRS) is a contagious viral infection and one of the most common infectious diseases of swine globally, responsible for significant economic losses worldwide. The infection was first recognized in the USA in 1987, while the first cases in Serbia occurred in 2001, after illegal import of boar semen (Petrović et al, 2011). The infection is caused by a single stranded RNA virus which belongs to the Arteriviridae family and the Arterivirus genus. The virus is biologically, antigenically and genetically heterogenic (Meng, 2000). PRRS virus is divided into two genotypes: PRRS-1 and PRRS-2 (ICTV - International Committee on Taxonomy of Viruses, 2018). Both genotypes are globally spread and enzootic in many countries. PRRSV-1 predominates in Europe while PRRSV-2 is most prevalent in Americas and Asia (Brar et al, 2015; Balka et al, 2018)
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