Comparison of Particulate, Block and Putty Forms of β-tricalcium Phosphate-Based Synthetic Bone Grafts on Rat Calvarium Model.

Abstract

Bone augmentation is a necessity for atrophied alveolar ridge prior to dental implant placement. Various bone graft types and forms with different characteristics are available in the market for alveolar augmentation. Beta tricalcium phosphate (β-TCP) is a synthetic biomaterial known as the oldest type of calcium phosphate. Studies comparing particulate, block or putty grafts are very limited. The aim of this study was to compare the particulate, block and putty forms of the same β-TCP bone graft and analyze the efficiency in critical size calvarium defects. Twenty male Wistar-Albino rats were employed for the study. Four bicortical bone defects with 5mm diameter were created on each rat calvarium, and three defects were filled with particulate, block or putty β-TCP graft and one defect was left empty. The animals were killed after 8weeks. New bone formation, residual graft, loose connective tissue, condensed mesenchyme, alkaline phosphatase, proliferating cell nuclear antigen, osteocalcin were measured on the specimens. Compared to block and putty forms, significantly higher new bone formation and least residual graft were observed in the particulate graft group. The residual graft was significantly higher in the block graft group than both the particulate and the putty groups. The cellular immunoreactivity of the samples in the particulate graft group was significantly higher. There was no significant difference between putty and block graft groups. Bone regeneration is significantly affected by the form of β-TCP bone graft, and the particulate form was the most successful in our study.