Abstract
Background: Trichomonas vaginalis is the most common cause of vaginitis. Diagnosis based on clinical presentation is often inaccurate.Clinical presentation with adjuvant laboratory evidence of trichomoniasis is required for the confirmation of the diagnosis and initiation of therapy.The aim of the present study was to compare the sensitivity and specificity of rapid staining Acridine Orange method for detection of trichomonas vaginalis infection with the routinely employed Papanicolaou staining.Methods: Vaginal swab smears were prepared from 300 patients attending gynaecology outpatients’ clinic of S.S.G. Hospital, Vadodara with complain of abnormal vaginal discharge. The prospective study was conducted over a period of two years (from January’ 98 to December’ 99). The Papanicolaou and Acridine Orange stained smears were screened for presence of Trichomonas vaginalis infection.Results: In the present study, the incidence of Trichomonas vaginalis infection was 12.3%. Considering Papanicolaou stain as standard, the sensitivity and specificity of Acridine Orange (AO) stain for detection of T. vaginalis was 92.8% and 93.01% respectively. The positive and negative predictive values were 74.2% and 99.2%, while false positive and false negative valuesare 3% and 0.66% respectively. Conclusion: Trichomonas vaginalis infection is a sexually transmitted disease of the reproductive age group, which needs to be timely diagnosed to prevent its adverse effects. The rapidity, ease and reliability of Acridine Orange stain justify its use in routine laboratory diagnosis of Trichomonas vaginalis infection. DOI: 10.21276/APALM.1592
Highlights
Trichomonas vaginalis is the most common sexually transmitted disease (STD) worldwide and affects an estimated 170-180 or more million people annually.[1,2] The WHO estimates an incidence of 276 million new cases each year.[3]
The rapidity, ease and reliability of Acridine Orange stain justify its use in routine laboratory diagnosis of Trichomonas vaginalis infection
There was disparity in 11 cases, in which 2 cases were positive by Papanicolaou stain and not by Acridine Orange, while 9 cases were positive by Acridine Orange but negative by Papanicolaou stain
Summary
Trichomonas vaginalis is the most common sexually transmitted disease (STD) worldwide and affects an estimated 170-180 or more million people annually.[1,2] The WHO estimates an incidence of 276 million new cases each year.[3] Trichomoniasis is almost entirely a disease of the childbearing era. There is no doubt that this infection is sexually transmitted. Trichomonas vaginalis is the most common cause of vaginitis. Diagnosis based on clinical presentation is often inaccurate. Clinical presentation with adjuvant laboratory evidence of trichomoniasis is required for the confirmation of the diagnosis and initiation of therapy. The aim of the present study was to compare the sensitivity and specificity of rapid staining Acridine Orange method for detection of trichomonas vaginalis infection with the routinely employed Papanicolaou staining
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