Comparison of Osmotic Resistance, Shape and Transmembrane Potential of Erythrocytes Collected from Healthy and Fed with High Fat-Carbohydrates Diet (HF-CD) Pigs-Protective Effect of Cistus incanus L. Extracts.

Sylwia Cyboran-Mikołajczyk,Dorota Bonarska-Kujawa,Michał Płóciennik,Robert Pasławski,Magdalena Wawrzyńska,Urszula Pasławska,Kacper Nowak,Paweł Kowalczyk,Katarzyna Męczarska,Jan Oszmiański

doi:10.3390/ma14041050

Abstract

Long-term high fat-carbohydrates diet (HF-CD) contributes to the formation of irreversible changes in the organism that lead to the emergence of civilization diseases. In this study, the impact of three-month high-fat diet on the physical properties of erythrocytes (RBCs) was studied. Furthermore, the biological activity of Cistus incanus L. extracts, plant known with high pro-health potential, in relation to normal and HF-CD RBCs, was determined. Obtained results have shown that, applied HF-CD modified shape, membrane potential and osmotic resistance of erythrocytes causing changes in membrane lipid composition and the distribution of lipids. The impact of HF-CD on physical properties of RBCs along with atherosclerotic lesions of the artery was visible, despite the lack of statistically significant changes in blood morphology and plasma lipid profile. This suggests that erythrocytes may be good markers of obesity-related diseases. The studies of biological activity of Cistus incanus L. extracts have demonstrated that they may ameliorate the effect of HF-CD on erythrocytes through the membrane-modifying and antioxidant activity.

Highlights

A diet enriched with saturated fatty acids and carbohydrates leads to reversible, than irreversible metabolic disturbances, which result in the emergence of civilization diseases, including obesity and accompanying diseases such as atherosclerosis and diabetes [1]
The results indicate no differences between hemolysis of high fat-carbohydrates diet (HF-CD)
The results have shown that RBCs treated with used compounds do not s

Summary

Introduction

A diet enriched with saturated fatty acids and carbohydrates (high fat-carbohydrates diet, HF-CD) leads to reversible, than irreversible metabolic disturbances, which result in the emergence of civilization diseases, including obesity and accompanying diseases such as atherosclerosis and diabetes [1]. The erythrocytes circulation is exposed to the free radicals due to their transporting functions in delivering oxygen to tissues. The RBCs are important in the progression and instability of atherosclerotic plaque They can accumulate into the atherosclerotic plaque after a plaque rupture or erosion and will increase the cholesterol content as well as the inflammatory/oxidative stress of the plaque [5]. The physical properties of the RBC membrane, and its cholesterol content, are of great importance in the development of atherosclerosis. Literature data indicate that the effects of HF-CD on RBCs depend on the length and composition of the diet and the research model used, the results obtained in various studies are often inconsistent.

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Conclusion