Abstract
As a primary aim, several approaches to photothermal (thermal-lens) and optoacoustic determination of exogenous pyrogens (lipopolysaccharides) based on photometric procedures for their determination at the microgram level and below are compared. The limits of detection of lipopolysaccharides by thermal-lens spectrometry and optoacoustic spectroscopy are at a level of 2-100 ng/mL, and the conditions of optoacoustic and photothermal procedures are the same. Optoacoustic spectroscopy is advantageous in determining suspensions, while thermal lensing is superior in determining lipopolysaccharides from homogenous aqueous solutions. As a secondary aim, photometric procedures for lipopolysaccharides by the formation of their ion pairs with several dyes and by the reaction of 2-keto-3-deoxyoctulosonic acid as a part of a lipopolysaccharide molecule with thiobarbituric acid are optimized. In the case of the 2-keto-3-deoxyoctulosonic acid reaction, the sampling stage time is decreased twofold, and the possibility of substitution of the toxic metaarsenite for sulfite with better sensitivity is shown.
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