Abstract
Background: The incidence of Dengue hemorrhagic fever, Dengue shock syndrome associated with Dengue can be reduced by diagnosing Dengue early and by initiating early treatment to Dengue patients. This study was conducted to compare results of NS1 antigen rapid test and ELISA in clinically suspected dengue patients.
 Materials and Methods: Present study was a comparative study conducted on 100 Patients presented with clinical history of Dengue. At Microbiology Laboratory, serum of all samples was assessed for NS1 detection using antigen Rapid test and ELISA. Sensitivity & specificity values were calculated for NS1 antigen rapid test, compared with ELISA.
 Results: Out of 100 serum samples collected from suspected cases of Dengue in and around Anantapuramu, 30 (30%) were positive for ELISA and 28 (28%) were positive for Rapid diagnostic test. Sensitivity & specificity when only NS1 was considered on rapid test kits when compared with ELISA were 93.33%, 98.57%,
 Conclusion: It is concluded that ELISA test was superior in the diagnosis of Dengue and recommended on improvement in sensitivity of RDTs.
Highlights
Dengue which is endemic in India is a, a mosquito-borne viral disease caused by infection with dengue virus (DENV 1-4) [1,2]
Many laboratories are following the methods like viral isolation, detection of viral genomic sequence by nucleic acid amplification technology assay (RTPCR), Antigen detection, nonstructural protein 1 (NS1) and the detection of dengue virus-specific IgM antibodies by the IgMcapture enzyme linked immunosorbent assay (MAC-enzyme-linked immunosorbent assay (ELISA)) and/or the rapid dengue immunochromatographic test (ICT) [4]
Out of total 100 samples, samples tested positive by NS1 antigen test were 28 (28%) and samples tested positive by NS1 ELISA were 30 (30 %) Table 2
Summary
Dengue which is endemic in India is a, a mosquito-borne viral disease caused by infection with dengue virus (DENV 1-4) [1,2]. Many laboratories are following the methods like viral isolation, detection of viral genomic sequence by nucleic acid amplification technology assay (RTPCR), Antigen detection, nonstructural protein 1 (NS1) and the detection of dengue virus-specific IgM antibodies by the IgMcapture enzyme linked immunosorbent assay (MAC-ELISA) and/or the rapid dengue immunochromatographic test (ICT) [4]. The present study was conducted to compare results of NS1 antigen rapid test and ELISA in clinically suspected dengue fever patients at a tertiary hospital. This study was conducted to compare results of NS1 antigen rapid test and ELISA in clinically suspected dengue patients. Materials and Methods: Present study was a comparative study conducted on 100 Patients presented with clinical history of Dengue. Results: Out of 100 serum samples collected from suspected cases of Dengue in and around
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