Comparison of Nathan alcohol formalin substitute fixative solution with 10% acetone-neutral buffered formalin in the preparation of cell blocks in ascitic fluid samples

Abstract

Cytology is one of the fields related to the study of the morphology of individual cells or cells derived from microscopically observed tissue fragments. Ascites is an abnormal collection of fluid in the peritoneal cavity. The peritoneal cavity contains 50-100 ml of serous fluid . In this study, the method used is the cell block method by doing a comparison between cell blocks fixed with NAFS and cell blocks fixed with acetone-NBF with an assessment of the quality of cytology preparations based on Hematoxylin-Eosin staining according to the criteria in the scoring table, namely scores 1 (not good), 2 (poor), and 3 (good). Good fixation will produce good quality preparations to be assessed by pathologists. The purpose of this study was to determine the comparison of fixative solution of nathan alcohol formalin substitute with 10% acetone-neutral buffered formalin in the manufacture of cell blocks in ascitic fluid samples. This type of research is quantitative research with experimental research design.