Abstract
Nanotechnology is employed in new and specific method for disease therapy. The shape and size of nano-particles are also important to serve the purposes. In this investigation, a comparison of inhibitory effects on Aspergillus niger and E. coli by nanosilver in 100, 200, 300, 400, and 500 ppm concentration was carried out. For this purpose, inseminated blank antibiogram disks were placed on bacterial culture over a nutrient agar environment of 0.5 Mac Farland`s standard. After an interval of first, second, and eighth days, the inhibitory zone was measured. In case of Aspergillus niger, PDA medium was used. The fungal growth was more sensitive to nanosilver than E.coli. Hence, nanosilver particles with 50, 100, and 150-ppm concentration were added to the culture medium for A.niger. After 6, 7, and 8 days, changes of growth, diameter and number of colonies were measured in treatment and control groups. In this investigation, nanosilver with 400-ppm concentration was more effective than the other concentrations for inhibiting E.coli. Effective time for induction of inhibitory effects on E.coli was 3-day post treatment with silver nano-particles. Effective dose of nanosilver concentration for growth inhibition of Aspergillus niger was 150ppm 8 day post treatment with silver nano-particles. Investigation of inhibitory effects for nanosilver concentration in Aspergilus niger and E. coli are examples of functional effects of new nano-biotechnlogy. These could be suggested in the therapy for human bacterial and fungal diseases. Keywords: Nanosilver, nanobiotechnology, Aspergilus niger, E. coli.
Highlights
Nanoparticles are small particles that have many radical-induced membrane and DNA damage
E. coli is one of physiologically would of new application for nano negative gram bacteria that are studied as a model of technology within the field of biotechnology
This paper highlights the comparison of nanosilver Materials and methods inhibitory effects on Aspergilus niger
Summary
Nanoparticles are small particles that have many radical-induced membrane and DNA damage. We specific physicochemical properties that may different study various aspects of nanoparticle on the growth effect from those of the bulk substance or particles of larger on fungus A. niger and bacterium E. coli Silver is one of these materials that have human beings, investigations on their growth inhibition antibacterial and antifungal effects. Investigations on specialized effects of silver negative and Gram-positive bacteria and antibiotic- nano particles for inhibiting fungus and bacterium resistant strains.
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