Abstract
Two monoclonal antibodies (MAbs) specific for a grapevine yellows mycoplasmalike organism (GY-MLO) were produced by fusing mouse myeloma cell lines NS1/1 to spleen cells of mice immunized with partially purified GY-MLO from diseased periwinkles. Using enzyme-linked immunosorbent assays (ELISAs) and indirect immunofluorescence (IF) staining, the MAbs were shown to be specific for the GY-MLO. Although both ELISA and IF tests were satisfactory with diseased periwinkles, serological detection of GY-MLO in diseased grapevines was not always reliable because of the extremely low titer of GY-MLO in the phloem and the interference of plant pigments in dot blot immunoassays [...]
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