Abstract
Biodegradation experiments using mixed bacterial cultures are difficult to compare because repeatable rates may not be produced in different experiments when cultures are normalized on a cell mass or number basis. A technique was therefore developed to quantify [14C]substrate-specific biodegradation activity of mixed cultures. The technique can be used to obtain a preliminary estimate of the substrate-degrading activity of a culture for more substantial experiments. The technique is applied to 1-naphthol-humic acid bioavailability experiments using both mixed and pure cultures to help isolate the impact of humic acid from differences between the cultures.
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