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Abstract
Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array. miRNAs target genes were predicted using miRror suite. Another independent mRNA expression profile dataset of 20 KBD patients and 15 healthy controls were integrated with the miRNA expression profiles of KBD. We identified 140 differently expressed miRNAs in KBD vs. Controls. GO enrichment analysis found that hypoxia, Wnt receptor signaling pathway and vitamin B6 biosynthesis related GO terms were significantly overrepresented in the target genes of differently expressed miRNAs in KBD vs. Control. 18 differently expressed common miRNAs were identified in KBD vs. Control, KBD vs. OA and KBD vs. RA. Integrating the lists of differently expressed miRNA target genes and mRNA differently expressed genes detected 6 common genes for KBD. Our results demonstrated the altered miRNAs expression profiles of KBD comparing to healthy controls, OA and RA, which provide novel clues for clarifying the mechanism of KBD.
Highlights
MicroRNAs are non-coding single-stranded small RNAs, which can regulate gene expression via complementarity to the 3′-UTR of Message RNA (mRNA) of target genes
Current Kashin-Beck disease (KBD) diagnosis is mainly based on clinical manifestations and radiographic changes of KBD
We provides the first evidence for altered miRNAs expression profiles of KBD comparing to healthy controls, OA and rheumatoid arthritis (RA)
Summary
MicroRNAs (miRNAs) are non-coding single-stranded small RNAs, which can regulate gene expression via complementarity to the 3′-UTR of mRNAs of target genes. Recent studies found that miRNAs played an important role in the joint injuries of OA and RA. Jones S.W. et al observed significant difference in miRNAs expression profiles between OA and healthy controls. No study has been conducted to investigate the potential roles of miRNAs in the joint injuries of KBD. Recent studies found that changes in miRNAs expression profiles were associated with multiple complex human diseases, such as OA and osteoporosis . We compared the miRNAs expression profiles of KBD, OA, RA and healthy controls. We observed altered miRNAs expression profiles of KBD comparing to healthy controls.
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