Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections.

Min Yi Wong,Bor-Shyh Lin,Yao-Kuang Huang,Tsung-Yu Huang,Yuan-Hsi Tseng,Chishih Chu,Chun-Wu Tung

doi:10.3390/microorganisms9010051

Abstract

Burkholderia cepacia complex (BCC) is a group of closely related bacteria with widespread environmental distribution. BCC bacteria are opportunistic pathogens that cause nosocomial infections in patients, especially cystic fibrosis (CF). Multilocus sequence typing (MLST) is used nowadays to differentiate species within the BCC complex. This study collected 41 BCC isolates from vascular access infections (VAIs) and other clinical infections between 2014 and 2020. We preliminarily identified bacterial isolates using standard biochemical procedures and further conducted recA gene sequencing and MLST for species identification. We determined genetic diversity indices using bioinformatics software. We studied 14 isolates retrieved from patients with VAIs and observed that Burkholderia cepacia was the predominant bacterial species, and B. contaminans followed by B. cenocepacia were mainly retrieved from patients with other infections. According to MLST data, we identified that all B. contaminans isolates belonged to ST102, while a wide variety of sequence types (STs) were found in B. cenocepacia isolates. In summary, the high diversity and easy transmission of BCC increase BCC infections, which provides insights into their potential clinical effects in non-CF infections.

Highlights

Introduction affiliationsBurkholderia cepacia was previously known as one bacteria species, but it has expanded to the Burkholderia cepacia complex (BCC), which comprises more than 20 closely related opportunistic pathogenic species [1,2]
Our six-year study autosomal recessive hereditary disease in the Caucasian population, is uncommon in Asia, collected 41 BCC isolates from non-cystic fibrosis (CF) patients that were from vascular access infections (VAIs) among hemodialysis with only a few cases published in Taiwan [24,25,26,27]
We noted that VAIs were more common than CF in isolates from non-CF patients that were from VAIs among hemodialysis patients and other

Summary

Introduction

Introduction affiliationsBurkholderia cepacia (formerly Pseudomonas cepacia) was previously known as one bacteria species, but it has expanded to the Burkholderia cepacia complex (BCC), which comprises more than 20 closely related opportunistic pathogenic species [1,2]. BCC is a group of genetically distinct but phenotypically similar bacteria that occur naturally in remarkably diverse ecological niches and possess extraordinary metabolic versatility [3,4,5]. Despite their agricultural potential, BCC is recognized among opportunistic nosocomial pathogens, with high potential to cause infections in patients with compromised immunity, cystic fibrosis (CF), chronic granulomatous disease, and indwelling medical devices [6,7]. Microorganisms 2021, 9, 51 a severe decline in lung function, possibly further developing into cepacia syndrome.

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