Abstract
BackgroundMobilization of mesenchymal stem cells (MSCs) from the synovium was revealed using a “suspended synovium culture model” of osteoarthritis (OA). The pathology of rheumatoid arthritis (RA) differs from that of OA. We investigated whether mobilization of MSCs from the synovium also occurred in RA, and we compared the properties of synovial MSCs collected from suspended synovium culture models of RA and OA.MethodsHuman synovium was harvested during total knee arthroplasty from the knee joints of patients with RA (n = 8) and OA (n = 6). The synovium was suspended in a bottle containing culture medium and a culture dish at the bottom. Cells were harvested from the dish and analyzed.ResultsNo significant difference was observed between RA and OA in the harvested cell numbers per g of synovium. However, the variation in the number of cells harvested from each donor was greater for RA than for OA. The harvested cells were multipotent and no difference was observed in the cartilage pellet weight between RA and OA. The surface epitopes of the cells in RA and OA were similar to those of MSCs.ConclusionMobilization of MSCs from the synovium was demonstrated using a suspended synovium culture model for RA. The harvested cell numbers, chondrogenic potentials, and surface epitope profiles were comparable between the RA and OA models.
Highlights
Mobilization of mesenchymal stem cells (MSCs) from the synovium was revealed using a “suspended synovium culture model” of osteoarthritis (OA)
We have recently demonstrated a “suspended synovium culture model” in which MSCs from the synovium of patients with OA were mobilized into a noncontacted culture dish through culture medium [4]
The passage 0 cell numbers varied greatly among the rheumatoid arthritis (RA) samples depending on the donor, whereas these numbers were similar in the OA samples
Summary
Mobilization of mesenchymal stem cells (MSCs) from the synovium was revealed using a “suspended synovium culture model” of osteoarthritis (OA). We investigated whether mobilization of MSCs from the synovium occurred in RA, and we compared the properties of synovial MSCs collected from suspended synovium culture models of RA and OA. MSCs are found in the synovial fluid of patients with rheumatoid arthritis (RA), an autoimmune condition characterized by inflammation, usually in bilateral joints, and systemic features such as fatigue or fever [5,6,7]. An unanswered question is whether the synovium of patients with RA directly releases MSCs into the synovial fluid, as occurs in patients with OA, because the pathological conditions of RA and OA are different. The primary aim of this study was to evaluate the possible release of MSCs from the synovium in a suspended synovium culture model of RA. Since some properties of synovial MSCs may vary depending on disease etiology, a secondary aim was to compare the properties of synovial MSCs obtained from the suspended synovium culture models of RA and OA
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