Abstract
BackgroundJewel orchid is the common name of several orchid species which can be alike in morphological characteristics, but variable in medicinal properties. At present, two DNA barcode loci, namely, maturase K (matK) and ribulose 1,5-biphosphate carboxylase (rbcL), are intensively utilized for plant identification. However, the discrimination effectiveness of these loci is variable among plant species. This study was carried out to compare the identifying efficacy of these two loci on jewel orchid population collected throughout Vietnam. ResultsThe results revealed that 21 jewel orchid accessions studied were segregated into four different species with significant variations. The discrimination power of matK and rbcL markers in this jewel orchid study displayed different efficiency level. The rbcL gene has higher distinguishing potential than either matK gene alone or the combination of both genes. ConclusionThe findings of this project could provide valuable information that is necessary for classification, plant origin identification, breeding, and conservation program of jewel orchid in Vietnam.
Highlights
Jewel orchid is the common name of several orchid species which can be alike in morphological characteristics, but variable in medicinal properties
The purpose of this study was to evaluate the species resolution ability of maturase K (matK) and rbcL loci in 21 accessions jewel orchid collected in Vietnam
Their study reported that when using Basic Local Alignment Search Tools (BLAST), the specimen was identified as Chenopodium murale for both matK and rbcL gene with 100% sequence similarity
Summary
Jewel orchid is the common name of several orchid species which can be alike in morphological characteristics, but variable in medicinal properties. The term “jewel orchid” refers to several species of orchid of velvety brocade-like leaves with beautiful veins. They belong to a diverse plant group of Orchidaceae family which spread widely in tropical regions of Asia and Australia, and have high medicinal and economic values. Several jewel orchid species may share nearly similar morphological characteristics their economic and pharmaceutical values are very different. The current plant identification is still using the traditional classification method: rely on the morphological characteristics of leaves, flowers, and stems. Applying the incorrect species with different pharmaceutical compounds as herbal
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